Screening

Betazymes places a strong emphasis on high-throughput screening (HTS) as a critical engine for identifying novel enzymes and optimizing existing ones. Our state-of-the-art screening platforms are designed to rapidly analyze the activity and properties of vast libraries of enzymes or enzyme variants. By miniaturizing assays and employing automated robotic systems, we can efficiently test thousands to millions of samples, significantly accelerating the enzyme discovery and engineering process. This robust screening capability allows us to quickly pinpoint promising candidates with desired characteristics for a wide range of industrial applications.

Our HTS workflows at Betazymes are highly versatile and can be tailored to screen for a variety of enzyme properties, including catalytic activity, substrate specificity, stability under different conditions (temperature, pH), and tolerance to inhibitors. We utilize a diverse array of detection methods, including colorimetric, fluorometric, and luminescent assays, to quantitatively assess enzyme performance. Furthermore, our integrated data analysis pipelines enable us to efficiently process and interpret the large datasets generated from HTS campaigns, allowing for rapid identification of lead enzyme candidates for further development.

Beyond primary screening, we also employ secondary and counter-screening assays to further characterize promising enzyme hits and eliminate false positives. These more detailed assays provide deeper insights into enzyme kinetics, substrate preferences, and potential off-target activities. Our iterative screening and characterization process ensures that we select only the most robust and specific enzyme candidates for downstream development and application. This rigorous approach maximizes the likelihood of identifying high-performing biocatalysts that meet the specific needs of our partners.

Betazymes is continuously investing in and refining our screening technologies to stay at the forefront of enzyme discovery and engineering. We are exploring novel assay formats, detection methods, and automation strategies to further enhance the speed, sensitivity, and throughput of our screening platforms. Our commitment to innovation in HTS allows us to efficiently navigate the vast landscape of enzyme diversity and rapidly identify or engineer biocatalysts with tailored properties for a wide spectrum of industrial and research applications.